How They Work
The advanced technology of Drug Alert kits ensures a dependable and accurate result for both illicit (street drugs) and prescription drugs.
The Principle Behind, Drug Alert Test Kits.
The test panel is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against their respective drug conjugate for binding sites on their specific antibody.
During testing, a urine specimen migrates upward by capillary action. A drug, if present in the specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug protein conjugate and a visible colored line will show up in the test line region of the specific drug strip. The presence of a drug above the cut off concentration will saturate all the binding sites of the antibody. Therefore, the coloured line will not form in the test line region.
A drug positive urine specimen will not generate a coloured line in the specific test line region of the strip because of drug competition, whilst a drug negative urine specimen will generate a line in the test region because of the absence of drug competition.
To serve as a procedural control, a coloured line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.